(A) Plasmids are present in bacteria only
(B) Plasmids are present in all organisms
(C) Plasmids present in bacteria and phages
(D) Plasmids present in plants and animals
Category: Microbiology Mcqs
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Which of the following statement about a vector is correct?
(A) All vectors are plasmids only
(B) Plasmids, phages can be used as vectors
(C) Fungi can also be used as vectors
(D) Cyanobacteria can also be used as vectors
During recombinant insulin synthesis, the bond between insulin polypeptide and galactosidase can be removed by using:
(A) Cyanogen bromide
(B) Chymotrypsin
(C) Carboxy peptidase
(D) Amylase
Transfer of recombinant plasmid into E. coli needs:
(A) Heat treatment
(B) UV-rays treatment
(C) MgCl2 treatment
(D) lysis
Restriction endonucleases cut DNA at a specific site that is known as:
(A) Ligation site
(B) ori
(C) Restriction site
(D) Replication site
An ideal plasmid to be used for recombinant DNA technology must have:
(A) Minimum amount of DNA
(B) Relaxed replication control
(C) One recognition site for one restriction endonuclease
(D) All of these
E. coli is generally used for gene cloning because:
(A) It supports the replication of recombinant DNA
(B) It is easy to transform
(C) It is free from elements that interferes with replication and recombination of DNA
(D) All of these
Expression vectors are those:
(A) Produce protein products
(B) Used for genomic libraries
(C) Used for chromosome synthesis
(D) Used for finger printing