(A) Cleaving DNA with restriction endonuclease and joining with ligase
(B) Cleaving DNA with ligase and joining with endonuclease
(C) Cleaving and joining DNA with restriction endonuclease
(D) Cleaving DNA with restriction endonuclease and joining with polymerase
‘Thermal Cycler’ is used in the reaction:
(A) Enzyme linked immune-sorbant assay
(B) Ligation reaction
(C) Polymerase chain reaction
(D) Immobilization reaction
The unique feature of monoclonal antibody is that:
(A) It is specific to a single antigenic determinant of a single antigen
(B) It is non-specific
(C) It is specific to a few antigenic determinants
(D) Restricted growth
Which one of the following organism is used for the large scale production of recombinant insulin?
(A) Plasmodium
(B) Agrobacterium
(C) Rhizobium
(D) E. coli
cancerous / myeloma cell in hybridoma helps in:
(A) Continuous growth of hybridoma
(B) Production of antibodies
(C) Both “A” and “B”
(D) Neither “A” nor “B”
A hybridoma cell:
(A) Produces different types of antibodies against different types of antigens
(B) Produces only specific antibodies only against a specific an
(C) Produces different types of antibodies but only one type of antigen
(D) None of the above
The restriction endonucleases are called so because:
(A) They have a very restrictive or site specific endonuclease activity
(B) They cut DNA at a few restricted sites
(C) They restrict the entry of foreign DNA into the cell by cleaving the DNA due to endonuclease activity
(D) Their distribution is restricted to only some bacterial cells
Which technique would most likely to be used to produce a large number of genetically identical offspring?
(A) Cloning and in vitro culture
(B) Polymerase chain reaction
(C) Chromatography
(D) Electrophoresis