(A) Vector, desired gene
(B) Vector, desired gene, mRNA of desired gene, host, restriction enzymes, ligases
(C) Desired gene, host, vector
(D) Vector, desired gene, mRNA of desired gene, host
Restriction endonucleases recognize specific sequences on DNA called:
(A) Non-coding sequences
(B) Satellites
(C) Palindromes with rotational symmetry
(D) Tandem repeats
VNTR stands for:
(A) Variable nucleotide triplet repeat
(B) Variable nucleoside tandem repeat
(C) Variable nucleoside triplet repeat
(D) Variable number tandem repeats
In restriction endonuclease EcoR1, “E” stands for?
(A) Extraction
(B) The first letter of the genus in which it is present
(C) Endonuclease
(D) Endangered
Restriction endonucleases are also called:
(A) Molecular scissors
(B) Molecular stichers
(C) DNA synthesis
(D) Polymerases
The scientists who won Nobel prize for physiology for their discovery of restriction endonucleases are:
(A) Jacob and Monad
(B) Smith, Nathans and Arber
(C) Watson and Crick
(D) Alec Jaffreys and Milstein
The presence of Restriction endonucleases was postulated in 1960 by:
(A) Khorana
(B) Watson
(C) Crick
(D) Arber
Restriction endonucleases, when present in a host cell act on foreign DNA molecule and cleave them, but they do not act on host DNA molecule. It happens because:
(A) Restriction endonuclease cannot act on host DNA
(B) Host DNA is packed into chromosom
(C) Host DNA is methylated hence restriction endonucleases can’t act.
(D) Restriction endonucleases become inactive when they reach host DNA